Journal: bioRxiv

Article Title: A novel bioreactor technology for modelling fibrosis in human and rodent precision-cut liver slices

doi: 10.1101/331173

Figure Lengend Snippet: ( A-B ) Graphs show fibronectin (μg/ml) and hyaluronic acid (ng/ml) levels in the media of bioreactor-cultured hPCLS after 24 rest and then 24h or 72h culture ± fib stim (TGFβ1/PDGFββ) ± Alk5i, numbers on bars show fold change compared to 48h control. ( C ) Graphs show mRNA levels of collagen 1A1, αSMA and TIMP1 in hPCLS at t=0 and after 24h rest plus 72h culture ± fib stim ± Alk5i, numbers on bars show fold change compared to t=0. ( D ) Representative 100x magnification images of αSMA (left panel) and Picrosirius red (right panel) stained hPCLS from three different donor livers at t=0, and after 24h rest plus 72h culture (4-days) ± fib stim ± Alk5i. Scale bars equal 200 µm. ( E ) Representative 200x magnification image of Picrosirius red stained hPCLS form donor 4. ( F ) Graph shows the percentage area of picrosirius red stained tissue in hPCLS at t=0, and after 24h rest plus 72h culture ± fib stim ± Alk5i (96h total culture). Data are mean ± SEM in n=4 independent slice experiments. P values were calculated using an Anova with Tukey’s multiple comparisons test (* P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001).

Article Snippet: ELISA quantifications for rat COL1a1 (LS-F11152, LSBio, UK), human (E88-129) and rat (E110-125) albumin (Bethyl laboratories, UK), human fibronectin (DY1918-05, R&D systems, UK) and human hyaluronic acid (DY2089, R&D systems, UK) were performed as per manufacturer’s instructions.

Techniques: Cell Culture, Staining

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 1 Expression of HOXB7 and bFGF genes in A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3. (a) RNase protection analysis. Riboprobes (R) and protected fragments (P) are shown on the right, and molecular size (base pairs) markers (MWM) (pGEM4Z HpaII) on the left. b-actin expression is shown as internal control. (b) Northern blot analysis. b2 microglobulin expression is shown as internal control. (c) Western blot analysis of bFGF produced by A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3 cells

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Expressing, Control, Northern Blot, Western Blot, Produced

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 3 (a) Role of bFGF for SkBr3/HOXB7 cell growth in low serum. Growth kinetics of parental and HOXB7-transduced SkBr3 cells maintained in 10% or 1% FCS were compared. Cell growth was monitored over the indicated time intervals by methylene blue inclusion, as indicated in Materials and methods. (b) Eect of exogenous rbFGF on SkBr3 cell growth. Growth curves of cells maintained in 1% FCS plus dierent concentra- tions of rbFGF evaluated by methylene blue inclusion

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques:

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 4 Inhibition of SkBr3/HOXB7 cell proliferation upon treatment with 30 mM of bFGF antisense (a) or of sense (s) oligomers. Evidence of bFGF intracrine loop operating in cells kept in low serum. Mean+s.d. of three separate experiments is shown

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Inhibition